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Combined bisulfite restriction analysis
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Combined bisulfite restriction analysis : ウィキペディア英語版
Combined bisulfite restriction analysis

Combined Bisulfite Restriction Analysis (or COBRA) is a molecular biology technique that allows for the sensitive quantification of DNA methylation levels at a specific genomic locus on a DNA sequence in a small sample of genomic DNA. The technique is a variation of bisulfite sequencing, and combines bisulfite conversion based polymerase chain reaction with restriction digestion. Originally developed to reliably handle minute amounts of genomic DNA from microdissected paraffin-embedded tissue samples, the technique has since seen widespread usage in cancer research and epigenetics studies.〔 This paper had 576 references as of February 2–11, according to Scopus
==Procedure==


抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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